Aliquots of nucleic
acid
RNA and DNA are extracted from histologically verified
frozen specimens in Ukraine and Russia. Extraction is carried
out to internationally agreed SOPs. The CTB website is currently
under development to allow registered users to access the
Standard Operating Procedures. The Institutes in Ukraine and
Russia and the Coordinating Centre carry our detailed quality
assurance using Agilent Bioanalysers.
Cases on which aliquots of nucleic acid are available at the
current time. Please contact the CTB
Secretariat for more details.
| Path Panel |
No of |
Tumour |
Normal |
Metastasis |
| Diagnosis |
cases |
DNA |
RNA |
DNA |
RNA |
DNA |
RNA |
| Cancer |
361 |
342 |
331 |
329 |
307 |
37 |
51 |
| (of these PTC) |
(304) |
(288) |
(278) |
(281) |
(261) |
(39) |
(50) |
| Benign |
127 |
118 |
123 |
94 |
89 |
|
|
| (of these FA) |
(86) |
(78) |
(82) |
(59) |
(55) |
|
|
| Other |
7 |
7 |
7 |
7 |
7 |
|
|
| Totals |
495 |
467 |
461 |
430 |
403 |
37 |
51 |
Table updated 04.05.2009
Aliquots of DNA from blood are also available and
in the majority of cases these can be paired with samples
of RNA and DNA from tissue from the same patient. DNA is extracted
using Qiagen kits.
Serum
Aliquots of serum are also available. These are prepared to
an agreed SOP.
There is no fee payable for provision of aliquots of nucleic
acid or serum, but investigators will be asked to cover the
costs of transport of the samples.
Paraffin sections
Sections are available from routine formalin fixed,
paraffin embedded tissue and from tissue microarrays (click
here for information on tissue microarrays).
The CTB is required to recover the costs of provision of this
material.
Costs for paraffin sections from individual paraffin blocks
are as follows:
20 US$ for the first section from each block, 3US$ for each
subsequent section
|
